Journal: BioMed Research International

Article Title: Chronic Heat Stress Inhibits Immune Responses to H5N1 Vaccination through Regulating CD4 + CD25 + Foxp3 + Tregs

doi: 10.1155/2013/160859

Figure Lengend Snippet: CHS negatively influences the immune effects induced by formalin-inactivated H5N1 virus. Serum samples and T cells of three mice from CHS+Ag and TN+Ag groups were collected on indicated days postprimary immunization. (a) Antibody levels against the H5 subtype antigen were expressed by HI titer. (b) Ag-specific IgG, IgG1, and IgG2a were determined by ELISA. (c) T cells were isolated on day 21 and stimulated with specific antigen; cell proliferation was determined by MTS/PMS method. (d) T cells collected on day 21 were stimulated with a viral-specific antigen for 6 h in vitro , stained with different florescent antibodies, and analyzed by flow cytometry. The numbers in the box indicate the percentages of examined cytokines. Data are summarized in a bar graph to the right. Results shown are pooled from three independent experiments.

Article Snippet: After blocking, plates were incubated with the mice serum samples in a serial dilution at 37°C for 1 h. Then, a 1 : 2000 dilution HRP-conjugated goat anti-mouse IgG, IgG1, and IgG2a (Proteintech, USA) were added.

Techniques: Virus, Enzyme-linked Immunosorbent Assay, Isolation, In Vitro, Staining, Flow Cytometry

Journal: Scientific Reports

Article Title: Deletion of platelet-derived growth factor receptor β suppresses tumorigenesis in metabolic dysfunction-associated steatohepatitis (MASH) mice with diabetes

doi: 10.1038/s41598-024-75713-6

Figure Lengend Snippet: List of antibodies for flow cytometry.

Article Snippet: Isotype control , PE-Vio770 , Rat IgG2a , Miltenyi Biotec , 130-102-647 , 1:800.

Techniques: Cytometry, Control